By Bancroft W. D.

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At 50~ under a pressure of 360 MPa, the activity is more than 30 times greater t han the activity at normal conditions (20~ and atmospheric pressure). Elevated hydrostatic pressure has also been found effective in increasing the stability of ~-chymotrypsin agai ns t t h e r m a l d e n a t u r a t i o n . For example, at 55~ the enzyme is a l m o s t i n s t a n t a n e o u s l y i n a c t i v a t e d at a t m o s p h e r i c pressure whereas under a pressure of 180 MPa, the activity is retained during several minutes.

H. Mantsch, Biochim. Biophys. Acta 47 (1988) 139. A. Driscoll, J. Jonas, and A. Jonas, Chem. Phys. Lipids 58 (1991) 97. 8 P. Mariani, V. Luzzati, and H. Delacroix, J. Mol. Biol. 204 (1988) 165. 9 G. Lindblom and L. Rilfors, Biochim. Biophys. Acta 988 (1989) 221. M. Seddon, Biochim Biophys. Acta 1031 (1990) 1. W. F. C. Turner, E. M. Gnmer, Chem~ Phys. Lipids 57 (1991) 147. 12 R. -C. Pilgrim, Ber. Bunsenges. Phys. Chem. 93 (1989) 708. 13 M. Bfttner, D. Cell, U. Jacobs, and R. Winter, Z. Phys. Chem.

Biophys. Acta, in press. 26 E. Gratton and T. Parasassi, J. of Fluorescence 5 (1995) 51. R. Hayashi and C. V. All rights reserved. 29 High pressure sensing and adaptation in the deep-sea bacterium Photobacterium species strain SS9. D. H. Bartlett, E. Chi, and T. J. Welch Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California, San Diego, La Jolla, California, U. S. A. 1 MPa-adapted bacteria. Genetic studies in the deep-sea bacterium Photobacterium species strain SS9 have uncovered two membrane-associated signal transduction systems, one of which is required for the inverse regulation of two genes encoding outer membrane proteins at low and high pressure, and the other which is critical for barophilic growth.

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Adsorption of Ammonia by Proteins by Bancroft W. D.

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